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recombinant human igfbp7 k95r protein  (R&D Systems)


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    Structured Review

    R&D Systems recombinant human igfbp7 k95r protein
    Recombinant Human Igfbp7 K95r Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igfbp7/Recombinant+Human+IGFBP-rp1%2FIGFBP-7+(K95R)+Protein%2C+CF/pmc13046735-246-37-41
    Average 94 stars, based on 10 article reviews
    recombinant human igfbp7 k95r protein - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: Development and provisional validation of a multiplex LC-MRM-MS test for timely kidney injury detection in urine
    Article Snippet: Recombinant SLC22A2 (Cat. Nr. H00006582-P01) was from Abnova (Taipei, Taiwan). .. Recombinant nephrin (Cat. Nr. 9399-NN-050) and biotinylated polyclonal antibodies against human TIMP2 (Cat. Nr. BAF971), IGFBP7 (Cat. Nr. BAF1334), KIM-1 (Cat. Nr. BAF1750), NGAL (Cat. Nr. BAF1757), CXCL9 (Cat. Nr. BAF392), cubilin (Cat. Nr. BAF3700), TGF-β1 (Cat. Nr. BAF246) and nephrin (Cat. Nr. BAF4269), were purchased from R&D systems (Minneapolis, Mn, USA). .. Recombinant KIM-1 (Cat. Nr. LS-G97633) was from LS Biologicals (Seattle, WA, USA) and recombinant CXCL9 (Cat. Nr. ab151917) and 3 TGF-β1 (Cat. Nr. ab50038) were from Abcam (Cambridge, UK).

    Article Title: IGFBP2 secretion by mammary adipocytes limits breast cancer invasion.
    Article Snippet: .. For the exogenous protein inverse invasion screen, cells were treated with either PBS or one of the following recombinant proteins (5 μM): SPARC (R&D Systems, 941-SP), DKK1 (R&D Systems, 5439-DK), DKK3 (R&D Systems, 1118-DK), TFPI (R&D Systems, 2974-PI), TFPI2 (R&D Systems, 2545-PI), HHIP (R&D Systems, 9280-HP), PAI1 (R&D Systems, 1786-PI), IGFBP2 (R&D Systems, 674-B2), IGFBP7 (R&D Systems, 1334-B7), and GSN (Novus, H00002934-Q01). .. For assessment of the IGF-II pathway perturbation, the following were used: recombinant IGF-II (10 ng/ml; R&D Systems, 292-G2), anti–IGF-II (10 μg/ml; Sigma-Aldrich, 05-166), or immunoglobulin G1κ (IgG1κ; 10 μg/ml; Sigma-Aldrich, M7894).

    Article Title: IGFBP2 secretion by mammary adipocytes limits breast cancer invasion
    Article Snippet: .. For the exogenous protein inverse invasion screen, cells were treated with either PBS or one of the following recombinant proteins (5 μM): SPARC (R&D Systems, 941-SP), DKK1 (R&D Systems, 5439-DK), DKK3 (R&D Systems, 1118-DK), TFPI (R&D Systems, 2974-PI), TFPI2 (R&D Systems, 2545-PI), HHIP (R&D Systems, 9280-HP), PAI1 (R&D Systems, 1786-PI), IGFBP2 (R&D Systems, 674-B2), IGFBP7 (R&D Systems, 1334-B7), and GSN (Novus, H00002934-Q01). .. For assessment of the IGF-II pathway perturbation, the following were used: recombinant IGF-II (10 ng/ml; R&D Systems, 292-G2), anti–IGF-II (10 μg/ml; Sigma-Aldrich, 05-166), or immunoglobulin G1κ (IgG1κ; 10 μg/ml; Sigma-Aldrich, M7894).

    Injection:

    Article Title: Delayed Administration of IGFBP7 Improved Bone Defect Healing via ZO‐1 Dependent Vessel Stabilization
    Article Snippet: To investigate the effects of IGFBP7 or AT1001 on vessel permeability in vivo, nude mice ( n = 6 per group) were subcutaneously injected with a mixture of human umbilical vein endothelial cells (1 × 10 7 ; HUVECs) and Matrigel (0.1 mL; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) at 4 °C on ice to construct subcutaneous tumors. .. One day after inoculation, the experimental group animals were injected with IGFBP7 (10 μg kg −1 ; 1334B7, R&D Systems, MN, USA) or AT1001 (50 mg kg −1 day −1 ; MCE, Monmouth Junction, NJ, USA), whereas the control mice were administered IgG. .. Then, 4 days after inoculation, FITC‐dextran (70 kDa, Invitrogen, Carlsbad, CA, USA) was constituted in 0.1 mL at a concentration of 10 mg m −1 and administered via intravenous tail injection.

    Article Title: Delayed Administration of IGFBP7 Improved Bone Defect Healing via ZO-1 Dependent Vessel Stabilization.
    Article Snippet: The vascular response following injury is pivotal for successful bone-defect repair but constitutes a major hurdle in the field of regenerative medicine.. Throughout this process, vessel stabilization is crucial to provide an adequate nutrient supply and facilitate efficient waste removal.. Therefore, this study investigated whether promoting vascular stabilization improves bone defect repair outcomes.

    Control:

    Article Title: Delayed Administration of IGFBP7 Improved Bone Defect Healing via ZO‐1 Dependent Vessel Stabilization
    Article Snippet: To investigate the effects of IGFBP7 or AT1001 on vessel permeability in vivo, nude mice ( n = 6 per group) were subcutaneously injected with a mixture of human umbilical vein endothelial cells (1 × 10 7 ; HUVECs) and Matrigel (0.1 mL; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) at 4 °C on ice to construct subcutaneous tumors. .. One day after inoculation, the experimental group animals were injected with IGFBP7 (10 μg kg −1 ; 1334B7, R&D Systems, MN, USA) or AT1001 (50 mg kg −1 day −1 ; MCE, Monmouth Junction, NJ, USA), whereas the control mice were administered IgG. .. Then, 4 days after inoculation, FITC‐dextran (70 kDa, Invitrogen, Carlsbad, CA, USA) was constituted in 0.1 mL at a concentration of 10 mg m −1 and administered via intravenous tail injection.

    Article Title: Delayed Administration of IGFBP7 Improved Bone Defect Healing via ZO‐1 Dependent Vessel Stabilization
    Article Snippet: .. The experimental group was treated with IGFBP7 (160 ng mL −1 ; 1334B7, R&D Systems, MN, USA), whereas the control group was treated with an equal volume of complete medium, followed by incubation in a controlled environment at 37 °C and 5% CO 2 for 48 h. Then, the upper chamber was washed twice with PBS. ..

    Article Title: Delayed Administration of IGFBP7 Improved Bone Defect Healing via ZO-1 Dependent Vessel Stabilization.
    Article Snippet: The vascular response following injury is pivotal for successful bone-defect repair but constitutes a major hurdle in the field of regenerative medicine.. Throughout this process, vessel stabilization is crucial to provide an adequate nutrient supply and facilitate efficient waste removal.. Therefore, this study investigated whether promoting vascular stabilization improves bone defect repair outcomes.

    Incubation:

    Article Title: Delayed Administration of IGFBP7 Improved Bone Defect Healing via ZO‐1 Dependent Vessel Stabilization
    Article Snippet: .. The experimental group was treated with IGFBP7 (160 ng mL −1 ; 1334B7, R&D Systems, MN, USA), whereas the control group was treated with an equal volume of complete medium, followed by incubation in a controlled environment at 37 °C and 5% CO 2 for 48 h. Then, the upper chamber was washed twice with PBS. ..

    Article Title: Delayed Administration of IGFBP7 Improved Bone Defect Healing via ZO-1 Dependent Vessel Stabilization.
    Article Snippet: The vascular response following injury is pivotal for successful bone-defect repair but constitutes a major hurdle in the field of regenerative medicine.. Throughout this process, vessel stabilization is crucial to provide an adequate nutrient supply and facilitate efficient waste removal.. Therefore, this study investigated whether promoting vascular stabilization improves bone defect repair outcomes.

    Saline:

    Article Title: Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.
    Article Snippet: Total protein was run on 4–12% Bis-Tris NuPage Gel (Invitrogen, NP0323BOX) under reducing conditions at 120 V for 90min, protein was subsequently transferred to methanolactivated PVDF membrane at 75 V for 90 mins. .. Membranes were blocked in 5% (w/v) skim milk in Tris-buffered saline with Tween20 (TBST), after which membranes were stained for either MECR (Thermo Fisher Scientific, PA5-54555, RRID:AB_2643839, 1:250), GATA4 (Abcam, ab124265, RRID:AB_11000793, 1:1000), ISL1 (Abcam, 86472, RRID:AB_1951287, 1:1000), NKX2.5 (Santa Cruz Biotechnology, sc-14033, RRID:AB_650281, 1:250), MYH6 (Abcam, ab50967, RRID:AB_942084, 1:1000), SLC8A1 (Cell Signaling, 55075- 1-AP, RRID:AB_2881262, 1:1000), ACTN2 (Sigma, A7811, RRID:AB_476766, 1:1000), ACTC1 (Sigma, SAB5600071, 1:1000), ANKRD1/CARP (Millipore, MABS1228, 1:250), CRHBP (Sigma, HPA046120, RRID:AB_10959760, 1:1000), TNNT2 (Abcam, ab91605, RRID:AB_2050427, 1:5000), IGFBP7 (R&D systems, AF1334, RRID:AB_2264436, 1:200), CCDC80 (R&D Systems, AF3410, 1:1000) TNNI1 (Sigma, AV42117, RRID:AB_1858352, 1:1000), MYL4 (Abcam, ab231800, 1:1000) or actin (Millipore, MAB1501R, RRID:AB_2223041, 1:10000). ..

    Staining:

    Article Title: Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.
    Article Snippet: Total protein was run on 4–12% Bis-Tris NuPage Gel (Invitrogen, NP0323BOX) under reducing conditions at 120 V for 90min, protein was subsequently transferred to methanolactivated PVDF membrane at 75 V for 90 mins. .. Membranes were blocked in 5% (w/v) skim milk in Tris-buffered saline with Tween20 (TBST), after which membranes were stained for either MECR (Thermo Fisher Scientific, PA5-54555, RRID:AB_2643839, 1:250), GATA4 (Abcam, ab124265, RRID:AB_11000793, 1:1000), ISL1 (Abcam, 86472, RRID:AB_1951287, 1:1000), NKX2.5 (Santa Cruz Biotechnology, sc-14033, RRID:AB_650281, 1:250), MYH6 (Abcam, ab50967, RRID:AB_942084, 1:1000), SLC8A1 (Cell Signaling, 55075- 1-AP, RRID:AB_2881262, 1:1000), ACTN2 (Sigma, A7811, RRID:AB_476766, 1:1000), ACTC1 (Sigma, SAB5600071, 1:1000), ANKRD1/CARP (Millipore, MABS1228, 1:250), CRHBP (Sigma, HPA046120, RRID:AB_10959760, 1:1000), TNNT2 (Abcam, ab91605, RRID:AB_2050427, 1:5000), IGFBP7 (R&D systems, AF1334, RRID:AB_2264436, 1:200), CCDC80 (R&D Systems, AF3410, 1:1000) TNNI1 (Sigma, AV42117, RRID:AB_1858352, 1:1000), MYL4 (Abcam, ab231800, 1:1000) or actin (Millipore, MAB1501R, RRID:AB_2223041, 1:10000). ..



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